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Ulmer Screenplay by: S. Edgar G. With its urbane, neurotic hero, American Matchmaker looks ahead to the films of Woody Allen. None is more charming than American Matchmaker. Ulmer One of the most versatile and resourceful filmmakers in movie history, Edgar George Ulmer, worked in a bewildering variety of genres, countries, and languages. In , Ulmer returned to Hollywood. There, among many other low-budget genre films, he made the quintessential film noir, Detour in ; his last movies were produced in Europe.

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MiRNA expression profiling is gaining popularity because miRNAs, as key regulators in gene expression networks, can influence many biological processes and have also shown promise as biomarkers for disease. Technological advances have enabled the development of various platforms for miRNA profiling, and an understanding of the strengths and pitfalls of different approaches can aid in the effective use of miRNA profiling for diverse applications. We review here the major considerations for carrying out and interpreting results of miRNA profiling studies, as well as current and emerging applications of miRNA profiling.

Since their discovery 1 — 3 , microRNAs miRNAs have come to be recognized as critical regulators of gene expression in plants and animals. However, one miRNA may regulate hundreds of mRNAs and as a result have substantial effects on gene expression networks. Consequently, miRNA expression patterns can be especially rich in biological information, since variation in expression of hundreds of messenger RNAs may, to an extent, be reflected in the expression patterns of one or a few miRNAs that regulate them.

MicroRNA expression profiling has proven to be useful for identifying miRNAs that are important in regulation of a range of processes, including organismal development and establishment and maintenance of tissue differentiation. As a result, miRNA expression biomarkers are finding application in diagnosis of tissue differentiation state in cancers of unknown origin, and miRNAs are being investigated as reagents for re-programming of cell fate in stem cell applications.

For investigators studying mechanisms of gene regulation, measuring miRNA expression can inform systems-level models of gene regulation, especially when miRNA information is combined with mRNA profiling and other genome-scale data. This has led to considerable interest in development of miRNAs as biomarkers for diverse molecular diagnostic applications, including in cancer, cardiovascular and autoimmune diseases, as well as to development of miRNA-based methods for forensic analysis where highly sensitive measurement of these molecules may provide information such as the cellular composition of forensic samples, for example.

Accordingly, miRNA profiling has become of interest to investigators working in diverse research areas of biology and medicine. Here we define miRNA profiling as measurement of the relative abundance of a cohort of miRNAs, ranging from a group of several miRNAs of specific biological interest, to comprehensive profiling of all miRNAs in a given species typically numbering in the several hundreds. Profiling of such small RNA molecules poses some inherent challenges, but substantial technological advances in recent years have overcome many of these barriers and a wide range of approaches and platforms are now available for miRNA profiling.

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Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Many transposable elements TEs use highly specific mechanisms to direct their integration to sites in the host genome that lack coding information.

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Only the momentary introduction of a bit of visual earthiness, presumably meant to balance off the overall painterly elegance of the picture, hinders endorsement for younger viewers. Comic misunderstandings ensue when the young British gentlewoman of the title, pertly played by Anya Taylor-Joy, tries her hand at matchmaking. The principal object of her ill-judged efforts is her fortuneless friend Harriet Smith Mia Goth. Born out of wedlock, Harriet is a student at a nearby boarding school in which Emma takes an aristocratic interest.

In keeping with the consummately proper source material, serious emotions are usually conveyed by the subtlest of gestures and passions are generally kept under wraps. Yet, as though to burst the bubble of refinement, the adaptation introduces one scene in which we see George, from behind, being undressed by his valet and another in which Emma, seen in profile, lifts the back of her skirts to warm her bare posterior in front of a fireplace.

While far from racy, and probably true to the cruder flip side of early 19th-century life, these moments may give some parents pause. The film contains fleeting rear male and partial female nudity in a nonsexual context, an illegitimacy theme and one mild oath. Some material may not be suitable for children. Catholic News Service is a leading agency for religious news.

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Well-polished, smartly relevant fiction. Traditional southern Indian society clashes with fast-changing Western ways, in a debut collection of eight elegant, nicely developed stories. Sankaran has her finger on the economic pulse that motivates many of these striving young characters, all from the provincial city of Bangalore, to embrace modern technological changes at the peril of rupturing family and culture.

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Volcanic Hills Viticulture: The grape varieties used in this red blend are: This wine has been one of our best-selling reds since its first release in The Okanagan grape growing industry was blessed again, but this time the weather was very hot which led to a super ripe, early harvest. Touch of sweetness, balanced acidity with integrated light tannin makes this a real easy sipping red wine.

The Gamay Noir vines were originally planted in with an additional planting in Tasting Notes: Salmon pink in colour and bursting with aromas of strawberry, pomegranate, rhubarb, floral with hints of spice. The palate is a juicy blend of strawberry and cherry, with ripe cranberry, pomegranate and spice that linger on the finish. The palate is well balanced with vibrant acidity. Food Pairings: A great brunch, lunch and picnic wine.

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Contributing writers include local dancers, choreographers, arts administrators, teachers, students, and more. Please send all press releases and inquiries about becoming a contributing writer to sam lovedancemore. For the premiere of its 56th season, Ballet West is presenting a mammoth historic revival that also offers – and asks of audiences – something acutely new and different. All of the flexion, angularity, layering of bodies, and familiar archetypes were evidently present even then, as were the first inklings of an ethos that would someday define an entirely new approach to ballet.

These three one-act ballets are also an incredible view into the rich collaborative relationships fostered by the Ballets Russes, with opulent intricate costumes designed by Henri Matisse and Georges Rouault and scores by Igor Stravinsky and Sergei Prokofiev. While each ballet was a stunning and cohesive achievement in its own right, each is now also made exponentially more powerful and relevant both through juxtaposition and a new ethos of presentation. The sparks that made this program truly noteworthy were fueled by its endeavor to be not just a series of beautiful ballets rich in legacy and refined in craft but also an ambitious, eye-opening, and socially conscious course of study.

A bulk of this attention fell to the revival of Le Chant du Rossignol. The process was three-fold: a project of in-depth research and dialogue; concrete changes made in response to that work; and a campaign to provide enrichment, transparency, and accessibility for audiences. Following extensive research done by the restaging team of Millicent Hodson and Kenneth Archer, who recovered existing knowledge of the original production, Ballet West invited leaders of the Asian American community, including Phil Chan, of the organization Final Bow for Yellow Face, and local arts advocate Max Chang, into the rehearsal process to begin a dialogue and develop a path forward.

This was followed by further discussions with and feedback from local schools and the general public, prior to opening weekend. It was wonderful to see Ballet West show up for this challenging, complex, and necessary conversation. It is also of utmost importance to note that Ballet West is responding to a pressure and cultural shift in the landscape that has been ongoing for some time now. The momentum begun and directed by dancers of color like Chan and his partner at Final Bow for Yellow Face, the New York City Ballet dancer Georgina Pazcoguin, may be attributed to the force now pushing companies across the country to expel outdated, harmful, or appropriative choreography in recent years.

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Jean-Georges is still a four-star restaurant. That is all. Thank you for your time. On second thought, there may be a little more to say. In a room that was all right angles and Manhattan energy, men and women servers a rarity in formal restaurants then circulated with unfussy, level, American poise. Most of all there was the brilliant cooking, which was lighter, brighter and more embracing of other cultures than French food had any right to be. What we want from restaurants is changing, though, along with our notions of luxury.